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ciap2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc ciap2
    Ciap2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ciap2/c-IAP2+Rabbit+mAb/bio_rxiv__64898__2026__03__09__710628-232-33-34
    Average 95 stars, based on 181 article reviews
    ciap2 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: E3 ligase Skp2-mediated stabilization of survivin contributes to radioresistance.
    Article Snippet: The inhibitors, including MG132, cycloheximide (CHX), Necrostatin-1 (Nec1), z-VAD-FMK and Chloroquine (CQ) from Selleck Chemicals (Houston, TX). .. Antibodies against Survivin (#2808; IB: 1:1000; IHC: 1: 2000), Skp2 (#2652, IB:1:2000, IHC: 1:1000), γ-H2AX (#9718; IB: 1:1000; IF: 1: 800), p-CDK1 Thr161 (#9114; 1:1000), p-Akt Ser473 (#4060; IB: 1:1000; IHC: 1: 50), α-Tubulin (#2125; 1:5000), VDAC1 (#4866; 1: 3000), Bax (#14796; 1:1000), Akt (#4691; 1:2000), anti-mouse IgG HRP (#7076; 1:10000), Flag-tag (#8146; 1:1000), cleaved-caspase 3 (#9664; IB: 1:1000; IHC: 1: 2000), p-Wee1 Ser642 (#4910; 1:1000), p-Survivin T34 (#8888; 1:1000), cytochrome C (#11940; 1:1000), cIAP2 (#3130; 1:1000), cIAP1 (#7065; 1:1000), Ub-k48 (#8081; 1:1000), β-actin (#3700; 1:1000) were obtained from Cell Signaling Technology, Inc. (Beverly, MA). .. Antibodies against Ki67 (#ab15580; IHC: 1:2000) and FBXL7 (#ab59149; 1:1000) were purchased from Abcam (Cambridge, UK).

    Western Blot:

    Article Title: Inhibition of AKT enhances chemotherapy efficacy and synergistically interacts with targeting of the Inhibitor of apoptosis proteins in oesophageal adenocarcinoma
    Article Snippet: Cells were counted using a Countess (ThermoFisher Scientific, UK) and the mean count ± the standard error of the mean (SEM) from 3 independent experiments was calculated. .. Western blot analysis was carried out as previously described using antibodies targeting phospho-(S473)-Akt (Cell Signaling Technology Cat# 4060, RRID:AB_2315049), phospho-(T308)-Akt (Cell Signaling Technology Cat# 4056, RRID:AB_331163), pan- Akt (Cell Signaling Technology Cat# 2920, RRID:AB_1147620), PTEN (Cell Signaling Technology Cat# 9559, RRID:AB_390810), PARP (Cell Signaling Technology Cat# 9532, RRID:AB_659884), phospho-γ-H2AX (Cell Signaling Technology Cat# 9718, RRID:AB_2118009), cIAP1 (Cell Signaling Technology Cat# 7065, RRID:AB_10890862), cIAP2 (Cell Signaling Technology Cat# 3130, RRID:AB_10693298), XIAP (Cell Signaling Technology Cat# 2045, RRID:AB_2214866) and Survivin (Cell Signaling Technology Cat# 2808, RRID:AB_2063948). .. Mouse monoclonal antibodies were used in conjunction with anti-mouse IgG, HRP-linked Antibody (Cell Signaling Technology Cat# 7076, RRID:AB_330924).

    Article Title: Epstein-barr virus latent membrane protein 1 targets cIAP1, cIAP2 and TRAF2 for proteasomal degradation to activate the non-canonical NF-κB pathway
    Article Snippet: .. For immunoblot analysis, Cell Signaling Technology antibodies against cIAP1 (D5G9), cIAP2 (58C7), XIAP (3B6), TRAF1 (45D3), TRAF3 (#4729), TRAF5 (#D3E2R), FLAG (M2, #2368), V5 (#D3H8Q), NIK (#4994) and ubiquitin (P4D1, #3936) were used at 1:1000 for immunoblot analysis. .. Antibodies against TRAF2 (Proteintech 26846–1-AP), TRAF2 (Invitrogen SD205–06), HA (Abcam ab9110), and p100/52 (EMD Millipore #05–361) were used at 1:1000.

    Article Title: Epstein-barr virus latent membrane protein 1 targets cIAP1, cIAP2 and TRAF2 for proteasomal degradation to activate the non-canonical NF-κB pathway.
    Article Snippet: .. For immunoblot analysis, Cell Signaling Technology antibodies against cIAP1 (D5G9), cIAP2 (58C7), XIAP (3B6), TRAF1 (45D3), TRAF3 (#4729), TRAF5 (#D3E2R), FLAG (M2, #2368), V5 (#D3H8Q), NIK (#4994) and ubiquitin (P4D1, #3936) were used at 1:1000 for immunoblot analysis. .. Antibodies against TRAF2 (Proteintech 26846–1-AP), TRAF2 (Invitrogen SD205–06), HA (Abcam ab9110), and p100/52 (EMD Millipore #05–361) were used at 1:1000.

    Ubiquitin Proteomics:

    Article Title: Epstein-barr virus latent membrane protein 1 targets cIAP1, cIAP2 and TRAF2 for proteasomal degradation to activate the non-canonical NF-κB pathway
    Article Snippet: .. For immunoblot analysis, Cell Signaling Technology antibodies against cIAP1 (D5G9), cIAP2 (58C7), XIAP (3B6), TRAF1 (45D3), TRAF3 (#4729), TRAF5 (#D3E2R), FLAG (M2, #2368), V5 (#D3H8Q), NIK (#4994) and ubiquitin (P4D1, #3936) were used at 1:1000 for immunoblot analysis. .. Antibodies against TRAF2 (Proteintech 26846–1-AP), TRAF2 (Invitrogen SD205–06), HA (Abcam ab9110), and p100/52 (EMD Millipore #05–361) were used at 1:1000.

    Article Title: Epstein-barr virus latent membrane protein 1 targets cIAP1, cIAP2 and TRAF2 for proteasomal degradation to activate the non-canonical NF-κB pathway.
    Article Snippet: .. For immunoblot analysis, Cell Signaling Technology antibodies against cIAP1 (D5G9), cIAP2 (58C7), XIAP (3B6), TRAF1 (45D3), TRAF3 (#4729), TRAF5 (#D3E2R), FLAG (M2, #2368), V5 (#D3H8Q), NIK (#4994) and ubiquitin (P4D1, #3936) were used at 1:1000 for immunoblot analysis. .. Antibodies against TRAF2 (Proteintech 26846–1-AP), TRAF2 (Invitrogen SD205–06), HA (Abcam ab9110), and p100/52 (EMD Millipore #05–361) were used at 1:1000.

    Article Title: CARD14 signalosome formation is associated with its endosomal relocation and mTORC1-induced keratinocyte proliferation
    Article Snippet: .. The following antibodies were used: anti-FLAG (M2) (Sigma, #F1804, or #F3165); anti-tubulin (TAT-1); p-IKK1/2 (2078S); p-p105 (CST, #4806); RelA (CST, #8242); p-ERK1/2 (CST, #9101); p-p38 (CST, #4511); HOIP (Merck, #SAB2102031); HOIL-1/RBCK1 (Atlas Antibodies, #HPA024185); SHARPIN (Proteintech, #14626); TRAF2 (Abcam, #ab126758); TRAF6 (Abcam, #ab40675); BCL10 (SCBT, #sc-5273); MALT1 (SCBT, #sc-28246; CST, #2494); ABIN1 (Ubiquigent, #68-0002-100); A20 (CST, #5630S); DIABLO (CST, #D5S3R); cIAP1 (CST, #7065); cIAP2 (CST, #3130); TAK1 (CST, #4505S); IKK2 (CST, #2684); EpsinR (Thermo Fisher Scientific, #PA5-60308); AP2B1 (SCBT, #sc-58226); AP2A1 (Abcam, #ab128950); total ubiquitin (CST, #3933); M1-linked ubiquitin (Merck, LUB9, #MABS451); K48-linked ubiquitin (CST, #8081); p-TAK1 (CST, #4536); p-TAB2 (CST, #8155); p-TRAF2 (CST, #13908); p-A20 (CST, #63523); p-MKK4 (CST, #9156); p-MK2 (CST, #3007); p-MEK1/2 (CST, #9121); p-JNK (CST, #9255/9251); p-JUN (CST, #9261/9164); p-FRA1 (CST, #5841); p-ATF2 (CST, #9225); p-mTOR (CST, #2481/2488); p-p70 S6K (CST, #9234); p-eIF4B (CST, #3591); p-S6 Ribosomal (CST, #2215). ..



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    Image Search Results


    Chidamide regulated the proteins associated with glycolysis, ferroptosis and DDP sensitivity by down-regulating USP35. A549 cells with treatment of 0.8 µM chidamide and H460 cells with treatment of 3.2 µM chidamide or not were transfected with oe-NC or oe-USP35. A - D WB analysis ( A ) was administrated for protein examination of PKM2 ( B ), FPN ( C ), and BIRC3 ( D ) in A549 cells. E - H WB results ( E ) for PKM2 ( F ), FPN ( G ), and BIRC3 ( H ) in H460 cells. */# P < 0.05, **/##/&& P < 0.01, ***/###/&&& P < 0.001. * : compared to oe-NC group, # : compared to oe-USP35 group, & : compared to chidamide + oe-NC group

    Journal: BMC Cancer

    Article Title: Chidamide impedes glycolysis but increases ferroptosis and cisplatin sensitivity of lung cancer cells through downregulating USP35

    doi: 10.1186/s12885-025-14925-z

    Figure Lengend Snippet: Chidamide regulated the proteins associated with glycolysis, ferroptosis and DDP sensitivity by down-regulating USP35. A549 cells with treatment of 0.8 µM chidamide and H460 cells with treatment of 3.2 µM chidamide or not were transfected with oe-NC or oe-USP35. A - D WB analysis ( A ) was administrated for protein examination of PKM2 ( B ), FPN ( C ), and BIRC3 ( D ) in A549 cells. E - H WB results ( E ) for PKM2 ( F ), FPN ( G ), and BIRC3 ( H ) in H460 cells. */# P < 0.05, **/##/&& P < 0.01, ***/###/&&& P < 0.001. * : compared to oe-NC group, # : compared to oe-USP35 group, & : compared to chidamide + oe-NC group

    Article Snippet: The primary antibodies against USP35 (proteintech, Wuhan, China, 24559-1-AP), PKM2 (proteintech, 15822-1-AP), FPN (proteintech, 26601-1-AP), BIRC3 (proteintech, 24304-1-AP), β-actin (proteintech, 20536-1-AP) were incubated overnight at 4°C.

    Techniques: Transfection